Fig. 4.1.

The gene of interest is cloned in suitable vector. For expression of the cloned gene, the gene is with all the essential regulatory elements required for transcription of the gene. The gene is attached to the selective gene, which helps in the selection of the clones with gene of interest. The cells are screened for the synthesis of desirable product and then processed for large-scale production in the bioreactor with optimum condition for high yields