Fig. 1.

MYMIV-based viral amplicon with reporter gene GFP (VA/GFP). (a) The map of MYMIV-DNA “A” genome. The map shown on the left side of the panel depicts the positions of different ORFs and the location of CR-AC3 (functional replicon) used for the study. (b) Map showing the VA vector and its cloning sites. The engineered viral insert containing GFP as a marker was cloned in the pCAMBIA1391Z background. The circular episome that formed in the plant leaves due to replication-release following agro-inoculation is shown schematically using a curved arrow.