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Amplification program for one step RT-qPCR assay
| No. | Step | Temperature (°C) | Time |
|---|---|---|---|
| 1 | Reverse transcription | 48 | 45 min |
| 2 | Initial denaturation | 94 | 02 min |
| 3 | Denaturation | 94 | 15 s |
| 4 | Annealing | 62 | 15 s |
| 5 | Extension | 72 | 15 s |
| 6 | Repeat steps 3–5 for 35 cycles | ||
| 7 | Final extension | 72 | 05 min |
