| Loading concentration (pM) | 6 | 8 | 10 | 12 | 14 | 20 |
|---|---|---|---|---|---|---|
| 20 pM lib pool mix (μL) | 180 | 240 | 300 | 360 | 420 | 600 |
| Pre-chilled HT1 (μL) | 420 | 360 | 300 | 240 | 180 | 0 |
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