Fig. 2.

Assessment of ezrin protein expression knockdown induced by siRNA transfections. 3.6 × 10³ HeLa-F5 cells were seeded in wells of a 96-well plate. The cells were transfected twice with non-targeting or ezrin-targeting siRNAs. For each condition, cells from one well were lysed and analyzed for ezrin and GAPDH housekeeping protein content by Western blot. The Western blot shown here displays three independent replicates for either non-targeting or ezrin targeting siRNAs