. 2007 Nov 28;454:43–59. doi: 10.1007/978-1-59745-181-9_5

Table 2.

Primer Sequences for Group 2a and BCoV or Bovine-like CoVs

Primers	Targeted region	Primer sequence 5′-3′	Product size	References
Pancorona-specific^a	RdRp (RNA-dependent RNA polymerase) gene	Forward (IN-2 deg) GGGDTGGGAYTAYCCHAARTGYGA	452 bp	(4)
Pancorona-specific^a	RdRp (RNA-dependent RNA polymerase) gene	For one-step RT-PCR Reverse (IN-4 deg) TARCAVACAACISY-RTCRTCA	452 bp	(4)
Group 2-specific	Nucleoprotein gene	Forward (Gr2F) GAAGGCTCDGGAAR-GTCTG	298––304 bp^b	Vlasova and Saif, unpublished
For one-step RT-PCR	Nucleoprotein gene	Reverse (Gr2R) CCTCTYTTHCCAAAACACTG	298––304 bp^b	Vlasova and Saif, unpublished
BCoV-specific-1	Nucleoprotein gene	Forward (NOF) GCAATCCAGTAGTA-GAGCGT	729 bp	(28)
For RT-PCR	Nucleoprotein gene	Reverse (NOR) CTTAGTGGCATCCTT-GCCAA	729 bp	(28)
BCoV-specific-2	Nucleoprotein gene	Forward (NF) GCCGATCAGTCCGACC-AATG	406 bp	(28)
For nested-PCR	Nucleoprotein gene	Reverse (NR) AGAATGTCAGCCGGGG-TAG	406 bp	(28)

^aThe universal primers are modified from Ksiazek et al. (4) (seeNote 8) for RNA sample screening in our lab.

^bBand size varies from 298 bp for MHV and SDAV-Rat coronaviruses to 304 bp for others, depending on exact nucleoprotein gene sequence.