| Step | Temperature | Time |
| Heat activation | 94°C | 8 min |
| Thermal cycling (40 cycles) | ||
| Denaturing step | 95°C | 30 s |
| Annealing step | 50°C | 40 s |
| Extension | 72°C | 15 s |
| Final extension | 72°C | 2 min |
| Soak | 4°C | ¥ |
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