| Step | Temperature (°C) | Time |
| UNG treatment | 50 | 2 min |
| Reverse transcription | 60 | 40 min |
| Heat inactivation | 95 | 5 min |
| Thermal cycling (50 cycles) | ||
| Denaturing | 95 | 15 s |
| Annealing and extension | 55 | 1 min |
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