Reagent	Volume per reaction	Volume for N reactions	Final concentration
10× PCR buffer	5 μL	5 × N μL	1×
25 mM MgCl2, 25 mM	5 μL	5 × N μL	2.5 mM
dNTP, 10 mM	1 μL	N μL	0.2 mM
Forward primers, 10 μM	1 μL	N μL	0.2 μM
Reverse primers, 10 μM	1 μL	N μL	0.2 μM
DNA polymerase (5 U/μl)	0.2 μL	0.2 × N μL	1 U/reaction
Water	34.8 μL	34.8 × N μL	–
Total	48 μL	48 × N μL	–