Fig. 2.

Ang I processing to Ang-(1-7) and Ang II in sheep proximal tubules. ¹²⁵I-Ang I (AI) was incubated with 50 μg of proximal tubules membranes for 30 minutes at 37 °C and the metabolites identified by HPLC. Panel a: Quantification of the peptidase activities for ¹²⁵I-AII metabolism from the sheep proximal tubule membranes expressed as the rate of metabolism products formed (fmol/mg/min). Conditions: Control (no inhibitors); +AP,CYS,CHM-I (inhibitors for aminopeptidase, carboxypeptidase, chymase, cysteine proteases); +NEP-I (addition of neprilysin inhibitor); +ACE-I (addition of ACE inhibitor); +ACE2-I (addition of ACE2 inhibitor). Panel b: Metabolism pathway for Ang I to Ang-(1-7), Ang II and degradation products in sheep proximal tubules. Data shown are mean values; n = 5. (Adapted from Shaltout et al. [43])