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Ligation of adaptors to the target sequence: (A) HinP1-I and Mse-I adaptors. (B) the restriction sites specific for HinP1-I and Mse-I enzymatic cleavage. (C) The product of ligation of the adaptors to the target sequence. Incorporation of a single nucleotide in the region of cleavage result in a nonfunctional cleavage site.
