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. 2007 Nov 28;454:73–89. doi: 10.1007/978-1-59745-181-9_7

Table 3.

Colony PCR Thermocycling Scheme

Time
(min)		 Temperature Number of cycles
5 95°C 1 cycle
1 95°C
1 55°C 25 cycles
2 72°C
10 72°C 1 cycle
∞ 4°C 1 cycle