Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001;154:91–116. doi: 10.1385/1-59259-043-8:91

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Humana Press Inc. 2001

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

PMC Copyright notice

Fig. 7. — Assembly of homo-oligomeric connexons in a cell-free translation system. Reticulocyte lysates were supplemented with microsomes and programmed with αCx43 RNA, α₁Cx32 RNA, and without (−) RNA in control. After translation was full-length (fl) completed microsomes were harvested, lysed in nonionic detergent, and assembly of connexins was analyzed by hydrodynamic analysis on linear sucrose gradients. Gradients were fractionated from the bottom, and sucrose concentration, radioactivity, and connexin protein content was determined in each fraction. Radioactivity recovered from each fraction was plotted vs the sucrose concentration after subtracting the lowest counts from each fraction. 9S particles represent assembled connexons, while 5S particles represent unassembled connexin polypeptides. More than 30% of the connexin polypeptides were recovered as assembled hexameric gap junction connexons. (Reproduced with kind permission fromref. 15).