Table 11.1.
Manual methods of nucleic acid extraction and purifications for rapid real-time PCR assays
| Kit/manufacturer/homepage | Technologic principle | Specimen throughput | Specimen type |
|---|---|---|---|
|
High Pure Roche Applied Science |
Nucleic acid capture by glass fiber fleece immobilized in a special plastic filter tube and subjected to centrifugation | 24 samples in 1 h | Serum, whole blood, plasma, urine, stool, sterile body fluids, respiratory tract specimens, swabs (genital, dermal) |
|
QIAamp Qiagen |
Nucleic acid capture by silica gel membrane placed in tube column and subjected to centrifugation or vacuum conditions |
24 samples in 1 h for DNA 24 samples in 1.5 h for RNA |
Respiratory tract specimens, plasma, stool, serum, whole blood, urine, sterile body fluids, swabs (nasal, fecal) |
|
IsoQuick Orca Research |
Nucleic acid is partitioned into an aqueous phase and then precipitated with ethanol and resuspended in water or buffer |
24 samples in 1 h for DNA 24 samples in 2 h for RNA |
Plasma, whole blood, stool, respiratory tract specimens, sterile body fluids, swabs (dermal, fecal, genital) |
|
IsoCode Stix Schleichar & Schuell |
DNA bound to matrix and released by simple water and heat elution | Processed individually | Whole blood |
Reprinted with permission from Espy MJ et al. (2006) Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev 19(1):165–256