Table 1.

Standard Conditions for Producing a PFGE Agarose Gel

Agarose concentration	0.8 %	1.0 %
Buffer	0.5X TBE	0.5X TBE
Gel volume	100 ml	100 ml
Initial pulse time	0.1 sec	3.0 sec
Final pulse time	1.0 sec	30.0 sec
Duration	6–16 h	16–20 h
Voltage	6.0 V/cm	6.0 V/cm