Fig. 13.2.

MultiBac tool-kits. A variety of entry plasmids to integrate heterologous genes into the MultiBac baculoviral genome have been created since the introduction of the system in 2004, each with its own merits. Functional modules contained in the plasmids are listed (bottom). All plasmids are compatible with each other and can be used in various combinations to generate recombinant MultiBac baculoviral genomes for multiprotein expression and/or multigene delivery. Expression cassettes are in ‘BioBrick’ format and enable iterative multiplication