Fig. 4.10.
In vitro expression of autophagy by the green fluorescent protein (GFP)-tagged light-chain 3 (LC3) in the PM-17-treated AsPC-1 cells. 2 × 105 AsPC-1 cells were cultured with the BRMI-1640 growth medium for 24 h in 24-wells plate, transfected with GFP-LC3 expression plasmid (0.8 μg/well) with lipofectamine 2000 transfection reagent (2 μg/well). After 24 h the cells were treated with and without PM-17 (175 μg/ml saline) for 24 h, and the distribution (a) of fluorescence of GFP-LC3 under fluorescence microscope and a Western blotting picture (b) were investigated