Fig. 3.

Mild acid hydrolysis-fluorometric HPLC analysis. Mini Q anion exchange chromatography of α2→8-linked di/oligo/polyNeu5Ac-DMB. α2→8-Linked oligo/polyNeu5Ac was labeled with DMB and applied to a mini Q HR5/5 anion exchange column (1 mL, Cl⁻-form). The column was eluted with 5 mM Tris-HCl (pH 8.0) with a gradient from 0 to 0.3 M NaCl for 75 min and 0.3 M NaCl to 0.4 M NaCl for 120 min after 15 min wash. The elution was monitored by a fluorescence detector (set at wavelength of 373 nm excitation and 448 nm emission). Each peak is assigned from the order of elution, based on DP