Fig. 4.
HPLC profile in the fluorometric C7/C9 analysis. A typical elution profile of DMB derivatives of C7-analogues and authentic sialic acids (C9) on the fluorometric HPLC. Disialyl silalitols, 12.5 ng each of Neu5Acα2→8Neu5Acα2→8-Neu5Ac-ol and Neu5Gcα2→8Neu5Gcα2→8-Neu5Gc-ol, were subjected to the periodate oxidation/reduction/hydrolysis, DMB derivatization, and fluorometric HPLC on a TSK-gel ODS-120T column (250 × 4.6 mm i.d.). The column was eluted with acetonitrile/methanol/water (9:7:84 by volume) at 1.0 mL/min at 26°C. Elution profile was monitored by measurement of fluorescence: excitation, 373 nm; emission, 448 nm