Table 2.
Antigenic specificities and class of anti-oligo/polySia antibodies
| Name of antibody | Animal origina and immunoglobulin-typeb | Sia in oligo/polySia recognized | Specificity on DP |
|---|---|---|---|
| <Group I > anti-polySia antibody | |||
| H.46 | ho, poly, IgM | Neu5Ac | DP ≥ 8 |
| 735 | mo, mono, IgG2a | Neu5Ac | DP ≥ 11 |
| <Group II > anti-oligo + polySia antibody | |||
| 12E3 | mo, mono, IgM | Neu5Ac | DP ≥ 5 |
| 5A5 | mo, mono, IgM | Neu5Ac | DP ≥ 3 |
| 2-2B | mo, mono, IgM | Neu5Ac | DP ≥ 4 |
| OL.28 | mo, mono, IgM | Neu5Ac | DP ≥ 4 |
| 2-4B | mo, mono, IgM | Neu5Gc | DP ≥ 2 |
| kdn8kdn | mo, mono, IgM | KDN | DP ≥ 2 |
| 4F7 | mo, mono, IgG | Neu5Acc | n.d.d |
| <Group III > anti-oligoSia antibody | |||
| S2-566 | mo, mono, IgM | Neu5Ac | DP = 2e |
| 1E6 | mo, mono, IgM | Neu5Ac | DP = 2 |
| A2B5 | mo, mono, IgM | Neu5Ac | DP = 3 |
| AC1 | mo, mono, IgG3 | Neu5Gc | DP = 2–4 |
a ho horse, mo mouse
b poly polyclonal, mono monoclonal
cα2→9-linkage specific
d n.d. not determined
eNeu5Acα2→8Neu5Acα2→3Gal. Gal residue is required