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DC-SIGN induces internalization of viral glycoproteins. HeLa and HeLa-DC-SIGN cells are incubated for 2 h at 4°C with 35S-labeled hepatitis C virus (HCV) E2 glycoprotein. Cells are extensively washed to eliminate unbound material and incubated for 30 min either at 4°C or 37°C. Cells are treated with EDTA or mock treated to distinguish internalized (EDTA-resistant) from cell surface bound HCV-E2 glycoprotein (EDTA-sensitive).
