. 2020 Mar 27;8:164. doi: 10.3389/fcell.2020.00164

TABLE 2.

GC-FID characterization of fatty acid profile in DHA-H- and DHA-treated HEK293T cell cultures.

	Control	DHA-H (30 μM, 24 h)	DHA-H (100 μM, 24 h)	DHA (30 μM, 24 h)	DHA (100 μM, 24 h)
Fatty acid	Mean^a ± SEM^a	Mean^a ± SEM^a	Mean^a ± SEM^a	Mean^a ± SEM^a	Mean^a ± SEM^a
2-OH 22:6 n-3	0.00 ± 0.00	0.21 ± 0.06**	0.74 ± 0.09***	0.00 ± 0.00 NS	0.00 ± 0.00 NS
22:6 n-3	3.10 ± 0.21	2.99 ± 0.23 NS	3.11 ± 0.21 NS	23.80 ± 1.85***	42.01 ± 5.62***
21:5 n-3	0.09 ± 0.01	1.74 ± 0.25***	4.01 ± 0.43***	0.20 ± 0.04 NS	0.52 ± 0.11 NS
22:5 n-3	1.94 ± 0.14	1.78 ± 0.20 NS	1.91 ± 0.22 NS	3.21 ± 0.35**	3.50 ± 0.35***
20:5 n-3	2.01 ± 0.10	1.80 ± 0.13 NS	1.97 ± 0.13 NS	6.99 ± 0.49***	6.16 ± 0.96***
20:4 n-6	5.74 ± 0.29	5.32 ± 0.50 NS	5.54 ± 0.53 NS	5.53 ± 0.84 NS	5.06 ± 0.84 NS
18:2 n-6	1.48 ± 0.16	1.40 ± 0.20 NS	1.50 ± 0.22 NS	1.31 ± 0.04 NS	1.40 ± 0.14 NS
18:1 n-9	39.74 ± 1.89	35.84 ± 2.75 NS	39.72 ± 3.41 NS	22.71 ± 2.74**	18.20 ± 2.52***
18:0	32.19 ± 4.70	30.07 ± 1.70 NS	33.51 ± 1.27 NS	33.61 ± 0.50 NS	33.34 ± 0.52 NS
16:0	42.27 ± 2.36	40.26 ± 3.88 NS	45.36 ± 4.62 NS	46.67 ± 3.18 NS	46.60 ± 4.41 NS

^aFatty acid levels (mean ± SEM) are shown as nmol/mg of protein. Multiple statistical analysis was performed with one-way ANOVA & Tukey’s post hoc test: NS, Not Significant. * p < 0.05, ** p < 0.01, and *** p < 0.001 as compared with control condition.