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. 2020 Apr 3;15(4):e0226396. doi: 10.1371/journal.pone.0226396

Fig 2. Elane-genotype does not significantly alter the anti-self response or the AFC compartment.

Fig 2

(A-D) Serum anti-RNA (A), anti-Sm (B), anti-nucleosome (C), and rheumatoid factor (D) antibody titers at 17 weeks of age. (E) Numbers of Ig κ+ antibody forming cells (AFCs) per spleen as determined by ELISpot (left panel). Percentages of live cells that are TCRβ- CD44+ CD138+ intracellular κ+ AFCs in spleens (right panel). (F-H) Numbers of IgG1 (F), IgG2a (elane+/+ males n = 9, elane -/- females n = 17, elane +/+ females n = 11) (G), and IgM (elane+/+ males n = 9, elane -/- females n = 14, elane +/+ females n = 10) (H) AFCs per spleen as determined by ELISpot (elane-/- males n = 8; elane+/+ males n = 11; elane -/- females n = 19; elane +/+ females n = 13 mice per group unless otherwise indicated). Data representation and statistics are as in Fig 1 unless otherwise indicated. In panel E (right), bar graphs are represented as the mean with SEM and a two-tailed Welch’s T test was performed to determine statistical significance within each gender.