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. 2010:145–186. doi: 10.1007/978-3-642-04809-8_5

Table 5.2.

Introns affecting transgene expression in different plants

Intron Specificity Remark Reference
COX 5c-1 Arabidopsis Increased GUS expression level Curi et al. (2005)
COX 5c-2
Ubi7 Potato Ten-fold higher expression Garbarino et al. (1995)
Adh1 Maize 40–100-fold increase in expression Callis et al. (1987)
Act1 Arabidopsis High level of reproductive tissue expression Vitale et al. (2003)
RBCS2 Chlamydomonas reinhardtii Stable high-level expression Lumbreras et al. (1998)
reg A3 Volvox carteri Required for regA expression Stark et al. (2001)
regA5
STK Arabidopsis Intron-mediated promoter expression in ovules and septum Kooiker et al. (2005)
VRN-1 Wheat Essential for promoter activity Fu et al. (2005)
Ostub 16 Rice Required for maximum promoter activity Morello et al. (2002)
OsCDPK 2 Rice Required for promoter activity Morello et al. (2006)
PpAct 1 Physcomitrella patens 11–18-fold higher expression Weise et al. (2006)
PpAct 5
PpAct 7
Sh1 Maize 10–1,000-fold enhanced expression Maas et al. (1990, 1991)
Gap A1 Maize Required for full promoter activity Donath et al. (1995)
Actin 3rd intron Maize IME Luehrsen and Walbot (1991)
Hsp81 Maize IME Sinibaldi and Mettler (1992)
Act 1 Rice IME McElroy et al. (1990)
tpi Rice Required for promoter activity Xu et al. (1994)