Table 16.2.
Cordycepin determination using different methods
| Cordyceps species | Objectives | Cultivation mode | Methods | Results | References |
|---|---|---|---|---|---|
| Cordyceps sinensis | Characterization | Submerged (cultured in Potato Dextrose Broth, PDB) | Automatic amino acid analyzer, HPLC | Cordycepin found in both cultured and natural Cordyceps sinensis, Cordycepin was introduced as an indicator for Cordyceps from its mimic and counterfeit | Hsu et al. (2002) |
| Cordyceps sinensis | Separation and concentration determination | Submerged (shake flask) | Capillary Zone Electrophoresis (CZE) | Rapid, Simple and Sensitive system to quantify the cordycepin | Ling et al. (2002) |
| Cordyceps militaris | Cordycepin determination | Solid-state fermentation | Capillary Electrophoresis (CE) + UV detection, HPLC | Low running cost and short analysis time are obtained as advantages of this method | Rao et al. (2006) |
| Cordyceps sinensis | Quantitative and qualitative evaluation of cordycepin | Submerged | HPLC-ESI-MS/MS, PLE, SRM and SIM | Good selectivity, linearity, recovery, precision, short analysis time + LOQ and LOD in the ng/ml range | Fan et al. (2006) |
| Cordyceps sinensis | Determination of cordycepin | Submerged | Ultra-performance liquid chromatography (UPLC) | Fast determination of analytes in pharmaceutical products and biological fluids | Yang et al. (2007) |
| Cordyceps militaris | Cordycepin extraction from wastes, propose technique for purification and separation | Solid fermentation | Column chromatographic extraction method (CCE) | High effective extraction method, minimal volume of solvent consumption, energy-saving, simple, environmentally friendly and low operating cost | Ni et al. (2009) |
| Cordyceps kyushuensis | Optimal extraction of cordycepin | Submerged | Surface fluid extraction (SFE), HSCCC + HPLC | Considerable amount of cordycepin with purity of 98.5% was obtained | Ling et al. (2009) |
| Cordyceps militaris and Cordyceps sinensis | Cordycepin analysis in natural and cultured Cordyceps | Submerged | Capillary electrophoresis-Mass spectroscopy (CE-MS) | Cordycepin was found in both natural and cultured Cordyceps Militaris but solely detected in natural Cordyceps sinensis with very low content | Yang et al. (2009) |
| Cordyceps sinensis | Determination of cordycepin and its transformation product | Submerged | IP-RP-LC-MS | No transformation was found in both natural and commercial cultured Cordyceps sinensis | Yang et al. (2010) |
| Cordyceps sinensis | Determination of cordycepin | Submerged | Liquid chromatography-Mass spectroscopy (LCMS) | Cordycepin was recovered from 98.47–99.32% | Xie et al. (2010) |
| Cordyceps militaris | Separation and purification of cordycepin | Submerged | High-speed counter-current chromatography (HSCCC) | Cordycepin with purity of 98.6% and 91.7% was produced | Zhang et al. (2016) |
| Cordyceps militaris Mutant G81–3 | Correlation determination between adenosine and cordycepin | Surface-liquid culture of Mutant | High-energy proton beam irradiation | Significant overproduction of cordycepin at 0.48 g/l/day | Masuda et al. (2011) |
| Cordyceps militaris Mutant G81–3 | Cordycepin production using crystallization method | Surface-liquid culture of Mutant | High-energy proton beam irradiation | High level production of cordycepin at 14.3 g/l | Masuda et al. (2014) |
| Cordyceps militaris 3936 | Optimization of cordycepin extraction | Submerged cultivation broth | Solvent-Solvent extraction | High yield extraction (95%) | Tuli et al. (2014a) |