Factor	Relevance
Good microbiological laboratory practice (GMLP)	Protection of operators (teachers, researchers, scientists, students, and lab technicians)
Levels of practical works (levels 1, 2, 3, and 4)	Degree of risk of microorganisms culture; well-trained of teacher and technician; student age and discipline
Select the microorganisms (ACDP hazard group 1)	Cultures must present minimum risk when GMLP are followed
Source of cultures	Reliable and professional supplier or permitted environment samples
Type of investigations/activities	Ample containment of cultures; hands-on work
Composition of culture media	The uses of some culture media are planned for selecting the growth of pathogens, i.e., not in ACDP hazard group 1
Volume of cultures	Increased risk with increase in volume of liquid culture
Laboratory facilities	Suitable level of containment for hands-on microbiological work
Equipment	Adequate for purposes
Incubation conditions	Proper selecting for the growth of pathogens
Disposal of contaminated materials	Ensures the elimination of risk to others
Proficiency of lab technicians, researchers and teachers	Skill and proper training in practices and procedures applicable to the level of practical work (levels 1, 2, 3, and 4)
Student age and discipline	Suitable level of practical work; self-confidence in class discipline
Sources of competent advice	ASE*, CLEAPSS*, MISAC, NCBE, SSERC* (*members only)
Useful check list	CLEAPSS Laboratory Handbook, ASE Topics in Safety, 2nd edition (ASE 1988), pp. 34–37
Essential reference	ASE Topics in Safety, 3rd edition (ASE 2001)