Extended Data Figure 1. GSDME expression in human and mouse tumor cell lines.

a,b, Gsdme mRNA (a) and protein (b) levels of indicated mouse cancer cell lines assessed by qPCR, relative to Gapdh, and by immunoblot, respectively. c, GSDME expression relative to GAPDH in normal tissue and tumors from breast invasive carcinoma (BRCA) and colon adenocarcinoma (COAD) patients from The Cancer Genome Atlas (TCGA) compared to the qRT-PCR values for mouse cancer cell lines used in this study. P-values comparing normal tissue and cancer tissues were calculated using unpaired two-tailed Student’s t-test. d,f, Expression of mGSDME in EMT6 (d), CT26 (f) clones knocked out (KO) for Gsdme or control (Ctl) cells treated with nontargeting vector, assessed by immunoblot for GSDME. Actin serves as loading control. e,g Cell proliferation determined by CellTiter 96 in EMT6 (e) or CT26 (g) ctl and Gsdme KO cells (n=6 samples/group). h, Expression of hGSDME in SH-SY5Y clones knocked out for GSDME or control (Ctl) cells treated with nontargeting vector, assessed by immunoblot for GSDME. i, j, Expression of mGSDME in GSDME OE and empty vector (EV)-transduced B16 (i) or 4T1E (j) by immunoblot. k, Expression of hGSDME in GSDME OE and empty vector (EV)-transduced HeLa by immunoblot. Differences among multiple groups in (e,g) were analyzed by one-way ANOVA, using the Holm-Sidak method for multiple comparisons. P-values in (e,g) compare KO and Ctl cells. *** P<0.0001. Data are mean ± SD of three technical (a) or six biological (e,g) replicates. Data are representative of at least two independent experiments.