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Representative image settings used in the HEK293T cell experiments
| Condition | Filter set | Excitation wavelength (nm) | Laser power (%) | PMT (V) | Gain (x) | Offset (%) | Pinhole | Dwell time (μs/pixel) |
|---|---|---|---|---|---|---|---|---|
| Donor | GFP | 488 | 2 | 365 | 1 | 0 | 300 | 2.0 |
| Acceptor | mCherry | 543 | 10 | 480 | 1 | 0 | 300 | 2.0 |
| FRET | mCherry | 488 | 2 | 480 | 1 | 0 | 300 | 2.0 |
