Table 4.
Materials and reagents used for measuring FRET in brain slices
| Reagent or resource | Source | Identifier |
|---|---|---|
| Laboratory mice (C57BL/6N), both male and female mice (between 8–12 weeks of age) | Jackson Labs | Jack stock # 005304; RRID: IMSR_JAX:005304 |
| Fine borosilicate glass micropipettes | WPI | 1B100-4 |
| Artificial tears ointment | Bausch & Lomb | NDC 24208-785-55 |
| Isoflurane (gas) | Henry Schein Animal Health | NDC 11695-6776-2 |
| Low-voltage heating pad | Sunbeam | Cat no. 732500000U |
| Buprenorphine (0.3 mg/ml stock) | MWI | Cat. no. 060969 |
| 10% povidone iodine | Purdue Products L.P. | NDC 67618-150-09 |
| 70% alcohol swabs | BD | HRI 326895 |
| Cotton swab and dental cotton balls | Richmond | Cat. no. 100110 |
| Nylon suture | Surgical Specialties | REF752B |
| Trimethoprim & sulfadiazine diet | Envigo | TD.06596 |
| AAV2/5 GfaABC1D NAPA-a SV40 | This manuscript, UPenn Vector Core | Available upon request from UPenn |
| AAV2/1 hSynapsin 1 Napa-n SV40 | This manuscript, UPenn Vector Core | Available upon request from UPenn |