Fig. 7.5.

Overview of a generic fermentation process for enzyme production in a fungal host (see main text for explanation). Typically, a defined medium is used and sterilized. A vial of the production strain is used for cultivation in an inoculation flask. Spores or cells are harvested after 5–10 days depending on the host and used to inoculate a seed fermenter. After 1–4 days, the culture is used to inoculate a larger tank where induction of enzyme production occurs by, e.g., feeding with an inducer. Both medium pH and temperature are controlled, the latter by, e.g., cooling water