Fig. 7.7.

Overview of generic approaches used for enzyme yield increase in fungal cell factories. A simplified model of a fungal tip is depicted showing the apical cell, the nucleus, the endoplasmic reticulum (ER), the Golgi apparatus, the secretory vesicles containing proteins to be secreted (green circles) that are transported to the hyphal tip through tubulin filaments (green lines), the chitosomes (red circles) containing enzymes involved in the synthesis of cell wall that are directed through actin filaments (red lines), and the Spitzenkörper, where secretory vesicles accumulate before exocytosis into the extracellular environment. Secretory vesicles can also be directed to vacuoles (v) for recycling. ∗Genome editing can be used both as a rational and high-throughput method for functional genomics analysis