Skip to main content
. 2020 Mar 6;161(5):bqaa039. doi: 10.1210/endocr/bqaa039

Figure 2.

Figure 2.

Stxbp1 demonstrates a circadian pattern of expression in mGLUTag L-cells that is regulated by BMAL1. mGLUTag L-cells were synchronized and analyzed for up to 48 hours by (A) RT-quantitative PCR for Stxbp1 and by (B) Western blot for STXBP1 and (C) BMAL1. (D-E) mGLUTag L-cells were analyzed by ChIP, at 8 and 20 hours following synchronization, for BMAL1 binding to the E-boxes at (D) -2016 bp and (E) -646 bp in the Stxbp1 promoter. ***P < 0.001; ##P < 0.01, ###P < 0.001 vs negative control (-IgG).