Fig. 2.

HPLC chromatograms showing separation of the products of the reaction of aminopeptidase M with Ac-Met-enk (detection at 280 nm). The products were separated isocratically by using 20% acetonitrile in 10 mM ammonium acetate at a flow rate of 1.5 ml/min. (a) Incubation for a period of 10 min—no reaction products are seen, only Ac-Met-enk elutes at 3.9 min. (b) Incubation period of 1 h—only 20% of the substrate is hydrolyzed. The peaks eluting at 2.0 and 3.9 min are due to Ac-Tyr and Ac-Met-enk, respectively.