Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional Information
Strain, strain background Mus musculus	Chx10-cre	Obtained from Jessell Laboratory (Crone et al., 2008)
Strain, strain background Mus musculus	tdTomato (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J)	Jackson Laboratories	Stock: 007914
Strain, strain background Mus musculus	ChR2-EYFP (B6.Cg-Gt(ROSA)26 Sortm32(CAG-COP4*H134R/EYFP)Hze/J)	Jackson Laboratories	Stock: 024109
Strain, strain background Mus musculus	GAD2-cre (B6N.Cg-Gad2tm2(cre)Zjh/J)	Jackson Laboratories	Stock:019022
Strain, strain background Mus musculus	TH-cre (B6.Cg-7630403G23 RikTg(Th-cre)1Tmd/J)	Jackson Laboratories	Stock: 008601
Strain, strain background Mus musculus	L7-Cre (B6.Cg-Tg(Pcp2-cre)3555Jdhu/J)	Jackson Laboratories	Stock: 010536
Recombinant DNA reagent	AAV2-EF1α-DIO-hChR2(H134R)-eYFP	UNC Viral Vector Core
Recombinant DNA reagent	AAV2-hSyn-hChR2 (H134R)-eYFP-WPRE-PA	UNC Viral Vector Core
Recombinant DNA reagent	AAVdj-hSyn-hChR2(E123A)-eYFP-WPRE	Stanford Viral Vector Core
Recombinant DNA reagent	AAV2-EF1α-DIO-eYFP	UNC Viral Vector Core
Chemical compound, drug	Cholera Toxin Subunit B (Recombinant), Alexa Fluor 488 Conjugate	ThermoFisher Scientific	Cat. No. C22841
Chemical compound, drug	Lumafluor red retrobeads	LumaFluor	Red Retrobeads IX
Chemical compound, drug	DNQX	Tocris	Cat. No. 0189
Chemical compound, drug	CPP	Tocris	Cat. No. 0247
Chemical compound, drug	CPCCOEt	Tocris	Cat. No. 1028
Chemical compound, drug	Sulpiride	Tocris	Cat. No. 0894
Chemical compound, drug	SCH 23390	Tocris	Cat. No. 0925
Chemical compound, drug	Prazosin	Tocris	Cat. No. 0623
Chemical compound, drug	Sotalol	Tocris	Cat. No. 0952
Chemical compound, drug	Quinpirole	Tocris	Cat. No. 1061
Chemical compound, drug	Dopamine	Tocris	Cat. No. 3548
Chemical compound, drug	Isoproterenol	Tocris	Cat. No 17.47
Software, algorithm	FreezeFrame	Actimetrics (www.actimetrics.com)