Fig. 3.

Expression of IFIT1 in response to siRNA transfection. MEF cells were transfected with Syk siRNAs A, B, C, G, H, I and J and the control siRNA against GAPDH and the expression of IFIT1 determined 2 days later using TaqMan PCR. IFIT1 expression was normalized to levels of RNA polymerase II mRNA and then expressed as the percentage expression compared to that in cell treated with the transfection reagent alone. Error bars represent the standard deviation of the mean for duplicate determinations from two separate experiments. A paired Student’s t-test was used to compare the statistical significance of the difference between data points. With the exception of Syk siRNAs C and H (marked with an asterisk), no significant increase in IFIT1 expression relative to the GAPDH siRNA control was observed with a p-value of less than 0.05.