Table 1.
Syk Knockdown and kinase inhibitors block TNFα expression and degranulation of histamine. RBL-2H3 cells were treated with the small-molecule inhibitors and siRNAs prior to activation of the FcεRI receptor with IgE and DNP-albumin as described in Section 2. The percentage release of TNFα and histamine compared to IgE/DNP alone is presented. For reference, the IgE/DNP positive control resulted in the release of 30 pg/ml TNFα and 21.3 nM histamine. Results are typical of two identical experiments.
| TNFα (% control) | Histamine (% control) | |
|---|---|---|
| No Treatment | 0 | 0 |
| IgE/DNP | 100 | 100 |
| BAY61-3606 1 μM | 0 | 0 |
| BAY61-3606 0.1 μM | 0 | 0 |
| R406 1 μM | 0 | 0 |
| R406 0.1 μM | 9 | 0 |
| Piceatannol 20 μM | 23 | 13 |
| Piceatannol 1 μM | 29 | 79 |
| siRNA A | 0 | 0 |
| siRNA G | 28 | 12 |
| siRNA J | 34 | 21 |