Table 3.

Summary of cytokine gene transcription in chicken splenocytes stimulated by the dsRNA molecules poly-UG and poly(I:C) after stimulation for up to 18 h.

Treatment	Hours	IFN-α	IFN-β	IFN-γ	TLR3	TLR7	IL-6	IL-1β	OAS
Poly-UG 50 nM	1	+	0	0	0	+	0	0	0
	3	0	0	0	0	0	0	0	0
	6	0	0	0	0	0	0	0	0
	12	+	+	0	0	0	+	0	+
	18	+	+	0	+	+	0	+	+
Poly(I:C) 50 ng/μL	1	+	+	−	−	+	+	+	+
	3	+	+	+	+	+	+	+	+
	6	+	+	+	+	+	+	+	+
	12	−	−	+	0	+	+	+	+
	18	0	−	+	0	+	+	+	+

0, no significant change; +, significant increase; −, significant decrease. Statistical significance at each of the time-points was evaluated by comparing the gene expression in the treatment group to that of vehicle (PEI-) controls.