Fig. 4.

Analysis of changes in the expression of proteins involved in iron-uptake pathways in P. aeruginosa cells grown in LB, CAA, or RPMI media. A, Proteomic analyses were performed on P. aeruginosa PAO1 cells grown over night in LB, CAA, or RPMI media. Average values measured in CAA or RPMI were plotted against average values measured in LB (two first panels) or CAA (third panels). Average values represent the average of the relative intensity of each protein, normalized against all proteins detected by shotgun analysis (n = 3). In red, proteins corresponding to TBDTs; in green, proteins other than FpvA of the PVD pathway; and in orange, proteins other than FptA of the PCH pathway. B–D, Heat maps of various TBDTs (B) and proteins involved in the PCH (C) and PVD (D) pathways: the darker the shade of green, the higher the expression of the protein is induced; the darker the shade of red, the stronger the expression of protein is repressed. Only the proteins for which a change in the level of expression was observed are shown. Supplemental Data S1 shows the detailed results of protein identification and quantitation.