Skip to main content
. 2020 Feb 26;19(4):655–671. doi: 10.1074/mcp.RA120.001955

Table I. S. cerevisiae strains used in this study.

Referred to as Genotype Name
pkaas+sch9as TB50a tpk1-M164G tpk2-M147G tpk3-M165G SCH9::sch9-T492G(pRS304) ypk1::Kan ypk2::HphNT pRS416-YPK1wt MPP88
pkaas TB50a tpk1-as tpk2-as tpk3-as SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-YPK1wt MPP86
sch9as TB50a Sch9::sch9-T492G(pRS304) ypk1::Kan ypk2::HphNT pRS416-YPK1-wt(791) MPP84
ypk1as TB50a SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-ypk1-L424G MPP91
pkaas+ypk1as TB50a tpk1-M164G tpk2-M147G tpk3-M165G SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-ypk1-L424G MPP87
wtas NTH1–3xFLAG TB50a SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-YPK1wt NTH1–3xFLAG::HIS3 yMJP012
pkaas NTH1–3xFLAG TB50a tpk1-M164G tpk2-M147G tpk3-M165G SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-YPK1wt NTH1–3xFLAG::HIS3 yMJP010
ypk1as NTH1–3xFLAG TB50a SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-ypk1-L424G NTH1–3xFLAG::HIS3 yMJP009
pkaas+ypk1as NTH1–3xFLAG TB50a tpk1-M164G tpk2-M147G tpk3-M165G SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-ypk1-L424G NTH1–3xFLAG::HIS3 yMJP040
wtas nth1–5A-3xFLAG TB50a SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-YPK1wt nth1-S20A,S21A,T58A,S60A,S83A-3xFLAG::HIS3 yMJP033
nth1Δ TB50a SCH9::SCH9wt(pRS304) ypk1::Kan ypk2::HphNT pRS416-YPK1wt nth1Δ::NatNT2 yMJP013