Fig. 5.

Design of histidine-rich peptides based on the molecular template of Ctry2459. (A) Sequence alignments of the WT, H2 and H3 peptides. The mutation sites are shown in green, and the histidine residues are shown in red. (B) Helical wheels of the three peptides showed the hydrophilic and hydrophobic faces. (C) CD spectra of the three peptides. Measurements were performed in the UV range of 250–190 nm at 25 °C in water on a Jasco-810 spectropolarimeter. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)