Fig. 4.

Knock-down of Bmptp-h reduces BmNPV replication in BmE cells. (A) BmE cells were transfected with dsRFP or dsBmPTP-h, and knock-down efficiency of Bmptp-h was measured by qPCR analysis. (B) 48 h after dsRNA transfection, cells were infected with BmNPV. Relative abundance of virus determined by BmNPV gp64 expression level was measured by qPCR analysis. (C) Fluorescence (GFP) and bright-field (BF) images of dsRFP- or dsBmPTP-h-treated cells at 72 h post infection of BmNPV-GFP. The mRNA level of target genes was normalized to the internal control (sw22934 or BmGAPDH) and represented as mean ± S.D. (n = 3). Statistical significance was assessed using Student's t-test. *p < 0.05. Scale: 50 μm.