Fig. 5.

Over-expression of Bmptp-h enhances BmNPV replication in BmE cells. (A) BmE cells were transfected with vector or Flag-BmPTP-h expressing plasmid 48 h before BmNPV infection. Relative abundance of virus determined by BmNPV gp64 expression level was measured by qPCR analysis. (B) Fluorescence (GFP) and bright-field (BF) images of vector- or BmPTP-h-transfected cells at 72 h post infection of BmNPV-GFP. The mRNA level of target genes was normalized to the internal control (sw22934 or BmGAPDH) and represented as mean ± S.D. (n = 3). Statistical significance was assessed using Student's t-test. *p < 0.05, **p < 0.01. Scale: 50 μm.