. Author manuscript; available in PMC: 2020 Apr 3.

Published in final edited form as: Methods Mol Biol. 2018 Jan 1;1757:69–113. doi: 10.1007/978-1-4939-7737-6_5

Table 4. Suggestions for User Comment Content.

Comment Type	Example comment
Gene name, including synonym	Purine Phosphoribosyl Transferase, is also known as HPRT, HGPRT, Hypoxanthine Phosphoribosyltransferase, Ppt1, Ppt-1, etc.
Reference	See PMID ##### for functional characterization of this gene." Same reference can be linked to multiple genes, if more than one gene is characterized in the manuscript.
Functional Characterization	This 'hypothetical protein' has been shown to be a copper transporter based on heterologous expression in Xenopus oocytes… Contact <xxxxx> for further details.
Subcellular localization	GFP tagging demonstrates that this protein localizes to the mitochondrion, as shown in the attached images. See attached image
Phenotype	Gene knockout has resulted in decreased growth… Contact <xxxxx> for further details
Structural information on annotated gene models	The predominant transcript initiation site for this gene has been mapped to ~561 nt upstream of the annotated ATG by 5'RACE and RNAse protection. This conclusion is consistent with available RNA-seq data, but differs from the reference annotation. See attached experimental evidence.