Table 1.

Performance of an “repaired” 7-year expired FD-DAT in comparison with non-expired freeze-dried (FD-DAT and liquid direct agglutination test (LQ-DAT) versions for the detection of VL

DAT version*	Number of serum with DAT titer: < 1:400 1:800 1:1,600 1:3,200† 1:6,400 1:12,800 ≥ 1;25,600
Repaired “expired” FD-DAT‡	3 4 2 1 2 3 2
Non-expired FD-DAT	2 6 1 1 2 0 5
LQ-DAT	6 1 2 0 1 2 5

DAT = direct agglutination; FD-DAT = freeze-dried direct agglutination test; VL = visceral leishmaniasis.

* No statistical difference between outcomes of the three DAT versions was found (Fr = 1.0588; P = 0.5890).

† Cutoff titer for VL.

‡ A 7-year expired FD-DAT batch repaired by substituting normal saline with citrate-saline/formaldehyde as anti-clumping/preservative solution.