Table 2.
Volumes of citrate-saline/formaldehyde used as antigen diluent in a reference FD-DAT against the corresponding promastigote concentration per unit suspension medium, the number of patients under investigation, and the application cost involved
| Anti-clumping/preservative volumes used for FD-DAT antigen reconstitution (mL) | Promastigote concentration/mL suspension | Antigen available for testing number of cases | FD-DAT application cost per vial |
|---|---|---|---|
| 5 | 9.0 × 107 | 8 | $32.0 |
| 6 | 7.8 × 107 | 10 | $25.6 |
| 7 | 6.5 × 107 | 11 | $23.3 |
| 8 | 5.4 × 107 | 13 | $19.7 |
| 9 | 4.7 × 107 | 15 | $17.0 |
| 10 | 3.4 × 107 | 17 | $15.0 |
| 11 | 2.3 × 107 | 18 | $14.2 |
| 12 | 1.4 × 107 | 20 | $12.8 |
| 13 | 0.8 × 107 | Invalid test outcome* | nd* |
FD-DAT = freeze-dried direct agglutination test.
* nd = Not estimated because of invalid test outcome.