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. 2020 Jan 4;102(4):863–875. doi: 10.1093/biolre/ioz232

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© The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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MYO6 forms a complex with TOM1/L2 and GIPC1 in mouse testes. (A) Immunoblotting of tissue extracts from sv/+ and sv/sv testes with anti-APPL1, anti-TOM1/L2, anti-GIPC1, and anti-actin antibodies. Actin is shown as a loading control. No changes in protein expression were observed in sv/sv spermatids in comparison to control cells. (B) Co-immunoprecipitation of MYO6, GIPC1, and TOM1/L2 from sv/+ testes lysates. MYO6 was immunoprecipitated with a rabbit polyclonal antibody. Lysates of sv/sv testes were used in the control lane. The immunoprecipitates were analyzed by western blotting with antibodies to GIPC1 and TOM1/L2.