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. 2020 Jan 9;102(4):975–983. doi: 10.1093/biolre/ioaa002

Figure 4.

Figure 4

The sterile phenotype of Tesmin KO male mice was rescued by a Tesmin-L transgene. (a) Upper: schematic representation of the Tesmin-L transgene. Black arrows: primer annealing sites. Lower: PCR genotyping to check the Tesmin mutation and transgene. The Tesmin mutation was checked with primer set a/b described in Figure 2a and the Tesmin-L transgene was checked with primer set F3/R2. (b) TESMIN-L expression in Tesmin +/+, +/em1, KO and KO mice carrying the Tesmin-L transgene was detected by western blotting. CALNEXIN was used as a control. (c) PAS–hematoxylin staining of the testis from Tesmin KO and KO mice expressing the Tesmin-L transgene. Scale bars: 50 μm. (d) Cauda epididymal spermatozoa from Tesmin +/+ and KO mice. Scale bars: 50 μm.