Figure 1.

Tcp11 is evolutionary conserved and expressed in testis. (A) Multiple sequence alignment of human, mouse, and rat TCP11. Darker color represents greater conservation. (B) Two verified variants of mouse TCP11 are annotated, one 567 amino acids long and a shorter version 488 amino acids long. Two additional TCP11 variants are predicted that are shorter. Most of TCP11 is composed of the TCP11 domain (black), which is specific to TCP11 homologs and is uncharacterized. Grey highlights the region specific to variant 1. Red highlights the region used to generate an antibody. (C) RT-PCR from various mouse tissues detects expression of Tcp11 in testis beginning at post-natal day 15. Hprt (hypoxanthine-guanine phosphoribosyltransferase) was used as a control.