Figure 2.

Tcp11-null males are subfertile. (A) Schematic of mouse Tcp11, the targeted Tcp11^tm1a(WTSI) floxed allele (Tcp11^fl), and the post-cre null allele Tcp11^tm1b (Tcp11⁻). (B) Genotyping PCR distinguishing wild-type, heterozygous Tcp11^+/−, and Tcp11 knockout (Tcp11^−/−) mice. (C) Western blot analysis with an antibody raised against a region near the N-terminus, detects both the longer and shorter variants of TCP11 in wild-type testis lysate but not in epididymal lysates. IZUMO1 (a component of the acrosome) confirms the presence of spermatozoa in both the testis and epididymal lysate. (D) Mating tests show Tcp11^−/− sire fewer pups over a three-month mating period than controls when paired with wild-type females (n = 5 males).