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TCP11 appears to not be present in mature sperm. Sperm isolated from the cauda epididymis were used for protein extraction into a Triton X-100- (membrane bound and cytoplasmic soluble), SDS- (axonemal components), and SDS-insoluble (fibrous sheath and outer dense fibers) pool. Western blot analysis against BASIGIN, acetylate-Tubulin, and AKAP4 demonstrates successful extraction of these three pools, respectively. Anti-TCP11 antibody only detects bands in the wild-type testis lysate.
